Dynamic Mechanism of Proton Transfer in Mannitol 2-Dehydrogenase from Pseudomonas fluorescens: MOBILE GLU292 CONTROLS PROTON RELAY THROUGH A WATER CHANNEL THAT CONNECTS THE ACTIVE SITE WITH BULK SOLVENT*

摘要

Background: The active site of mannitol 2-dehydrogenase from Pseudomonas fluorescens is connected with bulk solvent through a narrow protein channel. Glu292 adopts flexible positions down and up the channel.